To explore the mechanism of Sijunzi Decoction in the treatment of Alzheimer's disease (AD) based on network pharmacology.The effective components and target genes of Sijunzi Decoction were screened by TCMSP da-tabase and Uniprot database.The target genes of Alzheimer's disease were screened by Dragbank,Dis Ge NET and TTD databases.After mapping the component target with the disease target point,Cytoscape 3.7.1 software is used to construct the drug active component-target protein interaction network,and at the same time,the String database is used to draw the target protein-target protein interaction network;The target protein was analyzed by GO and KEGG using Metascape database.Finally,fluorescence real-time quantitative PCR was used to verify the main analysis results of network pharmacology.The results showed that Sijunzi Decoction was mainly associated with β-amyloid aggregation,apoptosis,inflammation,oxidative stress,autophagy,insulin metabolism etc.of AD.In vitro experiment showed that Sijunzi Decoction was involved in the regulation of APP-mediated β-amyloid aggregation,Caspase-3,Bcl-2,and Bax mediated apoptosis,MAOB mediated oxidative stress,mTOR mediated autophagy,and INSR mediated pathways of insulin metabolism.Conclusion demonstrated that Sijunzi Decoction has the characteristics of multi-component and multi-target treatment of AD,and which provided basis for further research on the mechanism of the decoction.