To investigate the hemostatic active compounds from the non-polysaccharide fraction of Bletilla striata(BS-80EE).UHPLC-ESI-Q-TOF MS was used to analyze the chemical constituents of BS-80EE.The hemostatic activities of compounds were evaluated through testing their effects on platelet aggregation induced by ADP and THR,and their CTs of heparinized mice.The results showed twelve compounds were identified from the BS-80EE.Among them,compounds 4-hydroxymethylphenyl β-D-glucoside (1),blestroside (5),gymnoside I (7),gymnoside Ⅸ (14) and armatuside (17) showed significant promoted platelet aggregation,and 4-hydroxymethylphenyl β-D-glucoside (1),α-isobutylmalic acid (3),blestroside (5),gymnoside I (7),militarine (8),gymnoside Ⅸ (14) and 1-p-hydroxybenzyl-4-methoxyphenanthrene-2,7-diol (18) could significantly shortened the CTs of heparinized mice.This results initially illuminated the hemostatic active ingredients of Bletilla striata.