A new isoflavones with ten known flavonoids were isolated from 70% ethanol extract of twigs of Ormosia hosiei by using various chromatographic techniques.Their structures were identified as 3′,4′,7-trihydroxy-5-methoxy isoflavone（1）,3′,4′,5,7-tetrahydroxy dihydroisoflavone（2）,genistin-5,4′-methyl ether（3）,lanceolarin（4）,biochanin A-7-O-rutinoside（5）,genistin（6）,apigenin-4′-O-β-D-glucoside（7）,vitexin（8）,prunin（9）,naringin（10）,neoeriocitrin（11）.Among them,compound 1 was a new compound，compound 2 was a new natural product.All compounds expect compound 6 were isolated from the plants of the genus Ormosia for the first time.In addition,the evaluation results of anti-inflammatory activity showed that the inhibited IL-6 secretion from LPS-induced BV-2 microglia by compounds 3, 4 and 9 were 37.67%±3.27%,36.02%±5.48% and 50.10%±1.58%,at the concentration of 20 μmol/L.