This work described the diarylpropane constituents from the ethyl acetate extracts of the fruits of Myristica fragrans and their anti-inflammatory activities.Five diarylpropanes were isolated through the combination of silica gel,RP-18,and Sephadex LH-20 gel column chromatography.Their structures were elucidated as 1-(3′，4′-dihydroxyphenyl)-3-(4″-methoxyphenyl)-propane (1),1-(4′-hydroxy-3′-methoxyphenyl)-3-(2″-hydroxy-4″-methoxyphenyl)-propane (2),horsfielenidine A (3),1-(2′-hydroxy-4′-methoxyphenyl)-3-(3″，4″-methylenedioxyphenyl）-propan-2-ol (4),and virolanol B (5) based on the comprehensive analysis of NMR (including ¹H,¹³C NMR (DEPT),HSQC,HMBC and 1H-1H COSY),HR-MS spectroscopic data,and the literature data.Compound 1 is a new diarypropane,which has not been reported.The in vitro nitric oxide (NO) production inhibitory tests of these compounds showed that compound 1 (IC₅₀ = 4.00 μmol/L) was more active than positive control NG-monomethyl-L-arginine,monoacetate salt (L-NMMA,IC₅₀ = 10.18 μmol/L) in LPS-induced RAW264.7 cells.This result showed potential for further investigation on anti-inflammatory diarylpropanes from M. fragrans.