In this research,we investigated the inhibitory effect of metabolites extracted from fermentation broth (MEs) of Bacillus subtilis BS-Z15 on Candida albicans,and discussed the effect of this extract on the treatment of C. albicans infected mice in Kunming.The minimum inhibitory concentration (MIC) of MEs was determined according to the "Antifungal Drug Sensitivity Test Protocol for Liquid-Based Dilution of Sporulating Filamentous Fungi".The therapeutic effect of MEs on the murine model of C. albicans infection in mice was analyzed using the following parameters:general condition,body weight,mortality,liver and spleen lesions,total antioxidant capacity (T-AOC),superoxide dismutase (SOD) activity,and malondialdehyde (MDA) content in serum.The results showed that the MIC of MEs against C. albicans was 0.33 mg/mL,and MEs could cause yeast C. albicans surface depression and lethality.Compared with the model group,mice treated with high doses of MEs showed better physiological status,significantly improved liver and spleen damage,significantly reduced mortality,significantly increased total antioxidant activity and SOD activity in serum,and significantly decreased MDA content.The comparison of MEs in mice treated with different doses showed that the treatment effect was dose-dependent in a certain concentration range.This indicates that B. subtilis BS-Z15 metabolites have a certain therapeutic effect on mice infected with C. albicans and have potential medicinal value in the treatment of C. albicans fungal diseases.