UPLC-Q-Exactive Orbitrap MS was used to establish the determination of salidroside,rutin,luteolin-7-O-glucoside,specnuezhenide, oleuropein,luteolin,apigenin,quercetin and echinacoside in Ligustri Lucidi Fructus simultaneously to compare and analyze the differences in the content of Ligustri Lucidi Fructus from different producing areas and before and after wine stewing.The Hypersll GOLD C₁₈ reverse phase chromatography column (100 mm×2.1 mm,3 μm) was used with a gradient elution of 0.1% formic acid solution and acetonitrile with the condition of electrospray ionization and negative ion mode.Combining chemical pattern recognition to analyze nine components changes of Ligustri Lucidi Fructus from different origins and before and after processing.The nine components have a good linear relationship within their respective concentration ranges (r≥0.999 6),and the average sample recovery rate is 97.4%-101.9%.The contents of nine components in Ligustri Lucidi Fructus from different habitats before and after processing are obviously different,of which specnuezhenide,salidroside,luteolin-7-O-glucoside,luteolin,rutin,oleuropein and echinacoside contribute greatly to the division of habitats;After processing,the contents of salidroside,specnuezhenide,oleuropein in Ligustri Lucidi Fructus increased significantly.The method is fast,accurate,highly sensitive,reproducible,and can provide a scientific basis for the perfect quality standards of Ligustri Lucidi Fructus and processed products.