To explore the hepatoprotective effect and mechanism of bergenin on acute liver injury induced by CCl₄ in rats based on IL-6/JAK2/STAT3 signal pathway.A total of 60 SD rats were randomly divided into normal group,model group,silymarin group (120 mg/kg),and bergenin low,middle and high dose (20,40,80 mg/kg) groups with 10 rats in each group.Rats in silymarin group and bergenin groups were given intragastric administration according to 10 mL/kg,while normal group and model group were given corresponding volume of solvent once a day for a week.Two hours after the last administration,except the normal group,the other groups were intraperitoneally injected with 25% CCl₄ olive oil solution 2 mL/kg,to establish the rat model of acute liver injury.After fasting,the blood was taken from the eyeball and the liver was collected after 16 hours of fasting.The levels or activities of alanine transaminase(ALT),aspartate transaminase(AST),albumin(ALB),malondialdehyde (MDA),glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in serum were determined by biochemical method.The contents of interleukin-6 (IL-6),tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in liver tissue were measured by enzyme-linked immunosorbent assay (ELISA).The pathological changes of liver tissue were observed by HE staining,and the expression level of IL-6 in liver tissue was observed by immunohistochemical (IHC).The protein expression levels of JAK2,p-JAK2,STAT3,p-STAT3,NF-κB p65 and p-NF-κB p65 were detected by Western blot.The results showed that inflammation,necrosis and IL-6 expression of liver tissue were reduced in different doses of bergenin and silymarin groups compared with model group,bergenin significantly reduced the activities or levels of ALT,AST,MDA in serum,TNF-α,IL-6 and IL-1β in liver tissue,up-regulated the activities or levels of ALB,SOD and GSH-Px in serum,and inhibited the expression of proteins related to IL-6/JAK2/STAT3 pathway.In conclusion,bergenin has a protective effect on acute liver injury induced by CCl₄ in rats,and the liver protection mechanism may be related to the inflammatory response and antioxidant mediated by IL-6/JAK2/STAT3 signaling pathway.