To investigate the chemical constituents of Dendrobium huoshanense Tang et Cheng.29 compounds were isolated from this plant by multiple column chromatography,such as silica gel,Sephadex LH-20,MCI gel,Rp-18 and semi-preparation HPLC,their structures were elucidated by means of extensive spectroscopic analysis and identified as follows:4,4′-dihydroxy-3,5-dimethoxybibenzyl (1),batatasin III (2),5,4′-dihydroxy-3-methoxybibenzyl (3),dihydroconiferyl dihydrop-hydroxycinnamate (4),methyl 3-propionate (5),dihydroconiferyl alcohol (6),dihydroferulic acid (7),4-O-β-D-glucopyranosyl coniferyl aldehyde (8),4-allyl-2,6-dimethoxyphenyl glucopyranoside (9),erythrosyringoylglycerol-4-O-β-D-glucopyranoside (10),3,4,5-trihydroxyallylbenzene-3-O-β-D-glucopyranosyl-4-O-β-D-glucopyranoside (11),(7S,8R)-syringylglycerol-8-O-4′-sinapyl ether 4-O-β-D-glucopyranoside (12),3,4-dihydroxy-5-methoxybenzaldehyde (13),p-hydroxybenzoic acid (14),5-hydroxylated isobenzofuran-1(3H)-one (15),3,5-dihydroxy-4-hydroxy benzaldehyde (16),4-hydroxy-3-methoxybenzaldehyde (17),3,4,5-trimethoxyphenol-1-O-β-D-glucopyranoside (18),gastrodin (19),(+)-syringaresinol (20),(+)-syringaresinol-4-O-β-D-glucopyranoside (21),liriodendrin (22),naringenin (23),2,6-dimethoxy-p-benzoquinone (24),5-hydroxymethyl furfural (25),tetrahydro-5-oxo-2-furancarboxylic acid methyl ester (26),methyl pyroglutamate (27),periloyrine (28) and uridine (29).Compounds 5-19,21-29 were isolated from this plant for the first time and 8,10,11,15,16,18,26,28 were isolated from this genus.