The ultrasound-assisted extraction process parameters of two active components (gentiopicroside,homoorientin) in Tibetan medicine Gentiana algida flower.To obtain the maximum of extraction ratio of the components.Three main factors including ethanol concentration,extraction time,extraction temperature were selected on the basis of experimental results of single factors.The optimum extraction condition were as follow:62% ethanol,extraction time 65 min,and extraction temperature 50 ℃.Under the conditions,the optimum extraction ratio of the two active components from G. algida flower was 0.75%,56%,close to the model prediction values （0.76% and 0.55%）.The results showed that the parameters were accurate,reliable and practical.