In order to study the anti-inflammatory effect of the Shengshao antisensitizer,the anti-inflammatory effect of the Shengshao antisensitizer was explored by exploring the activation level of calcineurin pathway and its effect on TNF-α-induced inflammatory response of HaCaT cells.Results showed that the same inhibitory effect as 125 μM tacromox (FK506) was observed at 1∶1 000.The amount of intracellular phosphorylation of P38 treated with TNF-α was decreased and increased with the decrease of the concentration of TNF-α,and 0.2% of them had the most significant effect.The Shengshao antisensitizer had a significant inhibitory effect on the nucleation phenomenon of NF-κB activation in cytoplasm induced by TNF-α stimulation,and the nucleation ratio decreases with the increase of concentration.Paeoniflorin and SymCalmin at the same concentration have the same effect.The Shengshao antisensitizer,paeoniflorin and SymCalmin could inhibit the intracellular expression of TNF-α after TNF-α stimulation,among which 0.05% of the Shengshao antisensitizer and paeoniflorin have the most obvious effects.The relative expression of IL-6 mRNA stimulated by TNF-α could be inhibited by the Shengshao antisensitizer,paeoniflorin and deminshu,among which,the effects of the Shengshao antisensitizer was decreased gradually with the increase of the concentration.The effect of the Shengshao antisensitizer was equivalent to that of paeoniflorin group,and that of deminshu group was more significant.The expression level of IL-8 mRNA stimulated by the Shengshao antisensitizer,paeoniflorin and deminshu was significantly decreased.The Shengshao antisensitizer,paeoniflorin and SymCalmin had no significant inhibitory effect on TNF-α induced IL-1α.The Shengshao antisensitizer,paeoniflorin and SymCalmin showed significant inhibitory effects on COX-2 expression induced by TNF-α.Therefore,the Shengshao antisensitizer has better anti-inflammatory effects.