A method for separation and determination of non-starch total polysaccharide in Mucuna pruriens var. utilis (MPT)was established. MPT was separated using water extraction followed by ethanol precipitation,size-extrusion column purification methods;monosaccharide composition of MPs was conducted by HPLC analysis of their PMP derivates.Three major constituents,MP-1,MP-2 and MP-3 were separated from MPT,with mannose,glucose and galactose as their monosaccharide compositions,respectively;The content of 3 monosaccharides in MPT was determined to be 9.69,7.87 and 12.09 mg/g,respectively.DNS colorimetry method was adopted to determine MPT amount in Mucuna pruriens var. utilis ,which was assayed to be 141.4 mg/g.MPT was composed of both MP-1 and MP-2 in major,and the determination methods mentioned above could be applied for quality analysis of MPT in Mucuna pruriens var. utilis .