To investigate the effects of drying process on the contents of eicosapentaenoic acid (EPA),α-linolenic acid (ALA),docosahexaenoic acid (DHA),arachidonic acid (ARA),docosapentaenoic acid (DPA),linoleic acid (LA) and oleic acid (OA) in Rana chensinensis ovum,the content of seven unsaturated fatty acids was determined by high performance liquid chromatography(HPLC)after natural drying,freeze drying and hot air drying (40,50,60 and 80 ℃).For different drying methods,the content of seven unsaturated fatty acids in Rana chensinensis ovum was 50 ℃ hot air drying > freeze drying > natural drying.The content of seven unsaturated fatty acids in Rana chensinensis  ovum at different temperatures of hot air drying was 50 ℃ hot air drying > 40 ℃ hot air drying > 60 ℃ hot air drying > 80 ℃ hot air drying.The results showed that different drying processes can affect the content of seven unsaturated fatty acids in Rana chensinensis  ovum to different degrees,with 50 ℃ hot air drying being the best drying process for Rana chensinensis  ovum,followed by freeze drying (loss rate 0.22%-5.64%),and then 40 ℃ hot air drying (loss rate 5.15%-7.39%).This study provided a reference for the processing and utilization of Rana chensinensis ovum.