To identify and analyze the main ingredients of Taraxacum mongolicum and to explore its therapeutic targets for cancer,HPLC-Q-TOF-MS/MS was used to analyze the extract in T. mongolicum. The active components of the T. mongolicum and the targets for anticancer were screened and predicted by SwissADME,Swiss target prediction and Genecards database.A target protein interaction network was constructed through the String online database,and the GO and KEGG enrichment analysis on key targets were performed by the DVIAD online database.Finally,a total of 29 compounds were identified from extract,including organic acids,flavonoids and other chemical components.Ten active ingredients were obtained,and 84 common targets for ingredients and diseases were screened.Further network analysis showed the main active ingredients including quercetin,luteolin,apigenin.The key targets were AKT1,EGFR,SRC,ESR1,PTGS2, MMP9,KDR,MMP2 and PIK3R1.In addition,the main process involved oxidation-reduction process,negative regulation of apoptotic process,protein autophosphorylation,ATP binding,protein kinase activity,protein serine/threonine kinase activity,enzyme binding,and the main pathway included pathways in cancer,proteoglycans in cancer,PI3K-Akt signaling pathway,etc.In summary,T. mongolicum plays a role in anticancer through multi-component,multi-target,and multi-pathways.