To study the chemical constituents from the whole herb of Acanthus lecucostachyus.Thirteen compounds (1-13) were isolated and purified by column chromatography on microporous resin,silica gel and SephadexLH-20.The structure of the compounds was identified by comparing their NMR,MS spectroscopic data with those in the literatures.These compounds were identified as 2-benzoxazolinone(1), 3-hydroxylacetyl-indole(2),catechol(3),vanillic acid(4),methyl 3,4-dihydroxy-benzoate(5),N-(2-hydroxyphenyl) formamide(6), carbazole(7),dibutyl phthalate(8),ethyl p-methoxy-trans-cinnamate(9),ethyl cinnamate(10),stigmasterol(11),3-hydroxy-4-methoxypyridine(12),stigmasterol-3-O-β-D-glucopyranoside(13).All compounds were isolated from this plant for the first time,in addition,compound 7 was found in the genus Acanthus for the first time.All isolates were evaluated for antioxidant activity by DPPH free radical scavenging method and anti-inflammatory activities by lipopolysaccharide (LPS) induced mouse monocyte macrophage RAW 264.7 to generate NO inflammatory model.Among those compounds,compound 3 showed moderate antioxidant activity with IC₅₀ value of 105.18 μM;Compounds 3,6 and 9 showed significant anti-inflammatory activity with IC₅₀ values of 18.49,22.84 and 41.22 μM,respectively.