To study the Chemical constituents from the ethyl acetate fraction of Gardenia jasminoides var. radicans.The compounds were separated by column chromatography such as silica gel,MCI gel CHP-20,ODS,and Sephadex LH-20 and purified by preparative HPLC method.Their structures were identified by spectral analysis and comparison with the data in reported literatures.Some of the isolated compounds were evaluated in vitro for their α-glucosidase inhibitory activity.As a result,seventeen compounds were isolated and their structures were identified as 5,3′,5′-trihydroxy-6,7,4′-trimethoxyflavone (1),5,7,3′-trihydroxy-6,4′,5′-trimethoxyflavone (2),5,7,4′-trihydroxy-6,3′,5′-trimethoxyflavone (3),astragalin (4),kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (5),kaempferol-3-O-robinobioside (6),episyringaresinol (7),medioresinol (8),zhebeiresinol (9),salicifoliol (10),methyl sinapate (11),methyl 4-hydroxy-3,5-dimethoxybenzenepropanoate (12),4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside (13),1-sinapoyl-O-β-D-glucopyranoside (14),2,2′-oxybis(1,4-di-tert-butylbenzene) (15),4-hydroxyphenylacetic acid (16),1-O-vanilloyl-β-D-glucose (17).Compounds 1,3,7,9-15 and 17 are isolated from genus Gardenia for the first time,and all the other compounds are isolated from this plant for the first time.The result of α-glucosidase inhibitory assay indicated that nine of the compounds exhibited slightly α-glucosidase inhibitory activity at the concentration of 100 μM.