To explore the application of Pueraria lobata in antioxidant and anti-aging field,two methods of fermentation and aqueous extraction were used to extract the active substances of P.lobata.The biochemical experiments were detected to evaluate the antioxidant activity of the extracts and Caenorhabditis elegans were used as model animal to detect the anti-aging effect.Results showed that 6.8% fermented broth of P. lobata (FBP) had better antioxidant ability than aqueous extract of P. lobata (AEP).The antioxidant ability included the total antioxidant capacity,the scavenging capacity of hydroxyl free radicals and superoxide anions.Compared with control group,FBP could significantly prolong the lifespan of C .elegans (P<0.05),and promote the fecundity of C. elegans by 4.8%.At the same time,the heat-resistant time of C. elegans was increased to 111.6% of the control group.Real-time quantitative PCR results showed that FBP could inhibit the expression of the pro-aging gene daf-2 and up-regulate the expression of the anti-aging gene daf-16.In addition,the FBP promote the expression of the downstream heat-resistant genehsp-16.2 and the antioxidant gene of sod-3.In conclusion,the FBP could prolong the lifespan of C. elegans by improving the antioxidant activity and heat resistance of the C. elegans.The good antioxidant and anti-aging effect of FBP provide a reference for its application in the field of anti-aging related food and cosmetics.