In order to discuss the effects of taurine on liver fat synthesis in high-fat diet rats,55 male SD rats were divided into 6 groups,regular diet control group and corresponding low/high-dose taurine groups,high-fat diet model group and corresponding low/high dose taurine groups.After 8 weeks feeding,high-dose taurine significantly reduced the body weight,epididymal fat index,serum triglycerides (TG),cholesterol (TC),ALT,AST,IL-4,TNF-α,and liver TG and TC of high-fat diet rats;and significantly reduced the mRNA and protein levels of SREBP-1c in high-fat diet rats liver,and also reduced the protein expression of SREBP-1c,AceCS1,FAS,ACC,p-IRS1-Ser302,p-mTOR-Ser2448,and increased the protein expression of p-ACC,p-PI3K -Tyr607,p-Akt-Ser473,p-Akt-Thr308.These results suggest that taurine can improve the inhibition of IRS1/PI3K/Akt pathway induced by high-fat diet,but its inhibition on SREBP-1c and subsequent intervention on rat liver fat synthesis were not regulated by this pathway.