The model of cisplatin-induced kidney cell damage was established based on in vitro experiment to investigate the attenuated mechanism of Glycyrrhiza uralensis endophyte on cisplatin-induced human embryonic kidney 293 (HEK293) cell damage.MTT assay was used to detect the effects of Glycyrrhiza uralensis endophyte,cisplatin and the combination of two drugs on the survival rate of HEK293 cells.The apoptosis rate of HEK293 cells was detected by flow cytometry,the mRNA and protein expressions of Bcl-2,Bax,Caspase-3,Caspase-8,Caspase-9 and endoplasmic reticulum stress pathway (GRP78,CHOP) were detected by Q-PCR and Western blot.The results showed that cisplatin induced HEK293 cell damage in a time-and dose-dependent manner.Glycyrrhiza uralensis endophyte could significantly reduce the apoptosis rate of cisplatin-damaged model by down-regulating the mRNA and protein expressions of Bax,Caspase-3,Caspase-8,Caspase-9,GRP78,and CHOP,up-regulating the mRNA and protein expression of Bcl-2 exerts a protective effect on cisplatin-induced renal cell injury.In conclusion.Glycyrrhiza uralensis endophyte could significantly reduce the apoptosis rate of cisplatin-damaged model,have anti-apoptotic effects on cisplatin-induced HEK293 cell damage,and the mechanism may be through regulation of apoptotic proteins of Bcl-2 family,Caspase family and endoplasmic reticulum stress pathway proteins to achieve anti-apoptotic effects and reduce cell damage.