To compare the alkaloid constituents of Corydalis from different habitats and explore the alkaloid constituents in Corydalis sheareri S.Moore.,the HPLC and acid dye colorimetry were used to compare the contents of protopine and total alkaloids in Corydalis from different habitats.Alkaloid constituents in Corydalis sheareri S.Moore. were identified by ultra-performance liquid chromatography-qua-drupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS).The results showed that the contents of protopine and total alkaloids in Corydalis from different habitats were not significantly different.A total of 13 alkaloids were identified by UPLC-Q-TOF-MS through high-resolution mass spectrometry data and relevant literature,including one rhoeadine-type alkaloid,one simple isoquinoline-type alkaloid,two protopine-type alkaloids,two protoberberine-type alkaloids,one phthalide-type alkaloid,four benzo-phenanthridine-type alkaloids,one tetrahydroproto-berberine-type alkaloid,one aporphine-type alkaloid. The results can provide a theoretical datum for the development of medicinal plant resources of Corydalis.