To study the mechanism of Huanglian Jiedu Decoction in the treatment of vascular dementia(VD) by network pharmacology.Searched the effective ingredients and related targets of Huanglian Jiedu Decoction by the TCMSP database,then obtained the targets of VD through the DisGeNET and GeneCards database.Constructed protein interaction network map by String online database,and to performed GO and KEGG enrichment analysis of key targets by R language.Induction of PC12 cell injury by adopted glutamate as VD cell model.Detection of cell viability by the MTT assay,and reactive superoxide dismutase (SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA) content by using Kits.ELISA and Western blot were used to verify the main biological processes and signaling pathways as predicted by network pharmacology.A total of 63 active ingredients of Huanglian Jiedu Decoction were screened,216 corresponding targets,105 targets related to VD,the top 20 KEGG related signaling pathways and GO analysis of the top 20 biological processes.Cell experiments confirmed that Huanglian Jiedu Decoction effectively increased the survival rate of glutamate-induced PC12 cells,inhibited the inflammatory response and oxidative stress response,reduced the protein expression of JNK,p-JNK,FoxO1,LC3Ⅱ/Ⅰ,Beclin1,Atg7,Caspase-3,Caspase-9,increased the protein expression of p-FoxO1 and P62.Huanglian Jiedu Decoction had a therapeutic effect on VD by inhibited the JNK/FoxO1 pathway,thereby inhibited inflammation,oxidative stress,autophagy and apoptosis.