To provide reference for quality control of Microctis Folium formula granules and related preparations,the quantity value transfer rule from Microctis Folium decoction pieces to Microctis Folium standard decoction was studied.The standard decoction was prepared from 15 batches of Microctis Folium decoction pieces.The paste rate and extracts of 15 batches of Microctis Folium standard decoction were measured and the fingerprints of Microctis Folium decoction pieces and standard decoction were established by ultra high performance liquid chromatography (UPLC).The common peaks were identified using Thermo Fisher QE high-resolution mass spectrometry and confirmed using reference materials.Using the relative peak area as a variable,orthogonal partial least square discriminant analysis (OPLS-DA) was performed on the fingerprints of Microctis Folium decoction pieces and standard decoction to find difference variables and three flavonoids in Microctis Folium standard decoction were determined.The results showed that the paste rate of 15 batches of Microctis Folium standard decoction ranged from 9.32% to 14.22% and the extracts ranged from 37.61% to 62.52%.The fingerprints of 15 batches of Microctis Folium decoction pieces and standard decoction were established with a similarity of more than 0.95.Twelve common peaks with consistent retention times were identified in the fingerprints of the decoction pieces and standard decoction and 10 chemical components were confirmed.OPLS-DA analysis found a total of 5 chromatographic peaks with significant changes in relative peak area,namely,peaks 1,2,3,4 and 6.All of them were phenolic acids and flavonoids.The contents of three flavonoids in 15 batches of standard decoction were within the mean ± 3 times SD range.The transfer rate of vitexin was the highest,followed by narcissin and isovitexin.This study can provide an important reference for the formulation of quality standards for Microctis Folium formula granules and related preparations.