In order to evaluate the correlation between flavonoid content and antioxidant activity in Nanfeng mandarin at different times,a method for simultaneous determination of 11 flavonoids by HPLC was established using Nanfeng mandarin from Nanfeng County as material.The antioxidant activity of Nanfeng Mandarin was evaluated by DPPH method and ABTS method,and a high performance liquid chromatography (HPLC) method for determining the content of flavonoids in Nanfeng Mandarin was established,which was relatively simple and efficient.The flavonoid composition and content of the peel and pulp of Nanfeng mandarin were very different,and it was found that the content of total flavonoids,chuanchen retinin and hesperidin,and the antioxidant activity could be evaluated by correlation analysis.This study provides an experimental basis for the antioxidant activity of Nanfeng mandarin mandarin and provides a scientific basis for its comprehensive utilization.