In order to maximize the potential of Olea europaea leaf resources and increase the variety of chemical components found in O. europaea leaves.In this study,the chemical makeup of O. europaea leaves was examined,and using silica gel column chromatography and other methods,12 flavonoids were extracted from the ethyl acetate portion of ethanol (95%) extract.Their structures were identified as apigenin (1),luteolin (2),rutin (3),apigenin-7-O-glucoside (4),quercetin (5),diosmetin-7-O-glucoside (6),diosmetin (7),kaempferol (8),taxifolin (9),luteoloside (10),isorhamnetin (11),epigallocatechin gallate (12),by ¹H NMR and ¹³C NMR,compound 12 was isolated from the leaves of O. europaea for the first time.The study has provided test basis for the further development and reasonable application of the O. europaea leaves.