This study was conducted to establish a method to determine the content of total flavonol glycosides in different parts of Meconopsis integrifolia from different habitats.The mobile phase consisted of methanol-0.2% formic acid buffer (48∶52,V/V),with a flow rate of 1.0 mL/min,using Megres C₁₈ column (4.6 mm ×250 mm,5 μm) with run time 40 min and UV detection at 254 nm.The column temperature was 40 ℃,and the injection volume was 5 μL.The results showed that the three flavonoids,quercetin,kaempferol and isorhamnetin,had good linear relationships in the concentration ranges of 0.7-70,0.7-70,and 0.4-40 μg/mL,respectively.The method is easy to operate,has good precision,stability and repeatability,and lays a foundation for further research on quality detection,development and utilization of M. integrifolia.