Pellinus lonicerinus (Bond.) Bond.et Sing is a Sanghuang variety commonly used in Hubei and other places,which is also the common medicine of Tujia ethnic,and polysaccharide is the main bioactive component of it.To study the extraction process and the antioxidant activity in vitro of polysaccharide from P. lonicerinus,the hot water extraction method was used in the experiment.The extraction process of polysaccharide from P. lonicerinus was optimized with multi-factor orthogonal test,based on the single factor experimental results.The antioxidant activity of five polysaccharides (PLP30,PLP60,PLP85,PLPA1 and PLPA2) was evaluated by five methods,namely reducing power,DPPH,ABTS and hydroxyl radicals scavenging activity,and the inhibition of tyrosinase activity.The results showed that the optimal extraction condition was:the ratio of material to liquid was 1∶20 g/mL,extraction time of 120 min,extraction temperature of 100 ℃.Under this optimized condition,the average extraction yield was (3.16±0.05)%.In vitro antioxidant activity tests showed that five polysaccharides had a certain reducing power and scavenging effect on DPPH free radical,ABTS free radical and hydroxyl free radical,the reducing power and ABTS radical scavenging effect had concentration-response relationship with its concentration.These results indicated that polysaccharides from the fruit body of P. lonicerinus had good antioxidant and tyrosinase inhibitory ability in vitro.