To explore the mechanism of G. pentaphyllum in the prevention and treatment of diabetes,this study employed network pharmacology and molecular docking methods to investigate the effective ingredients and pharmacological effects of G. pentaphyllum.Through databases such as TCMSP,SwissTargetPrediction,and GeneCards,20 active ingredients of G. pentaphyllum and 155 potential targets in the prevention and treatment of diabetes were retrieved and screened.Subsequently,using the STRING database,57 key targets were obtained through protein-protein interaction screening.GO enrichment and KEGG pathway analysis identified 20 biological processes,20 molecular functions,17 cell ingredients,and 20 major signaling pathways involving 12 active ingredients of G. pentaphyllum and 53 key targets.Molecular docking results indicated that 3′-methyleriodictyol and Rhamnazin exhibited lower binding free energy with key targets such as MAPK8 and PIK3CA.In conclusion,the main mechanism of G. pentaphyllum in the prevention and treatment of diabetes may involve regulating insulin and related cellular proliferation,apoptosis,and migration processes through signaling pathways such as PI3K-Akt and insulin resistance.