This study aims to enhance the extraction yield of total iridoid glycosides from Lagotis yunnanensis W.W.Smith and evaluate their in vitro anti-oxidant and anti-inflammatory properties.Based on the single factor experimental results, Box-Behnken response surface analysis was carried out by flash extraction method.The average extraction rate of total iridoid glycosides from L. yunnanensis was 10.56%.The total iridoid glycosides can better scour DPPH and ABTS free radicals and show anti-inflammatory effect on lipopolysaccharide-induced RAW 267.4 cells inflammation model, reduce the expression of NO and TNF-α in cells.The extraction process obtained in this study is simple, stable and reliable.It can be used for the further research and application of total iridoid glycosides of L. yunnanensis.