The aim of this study was to establish a mouse model of chronic interstitial kidney injury induced by a high adenine diet.Twenty-four week-old C57BL/6J male mice were given adenine by gavage and adenine-containing diet.The experiment period was eight weeks,including induction period,transformation and development period and intervention period.The evaluation indexes of the model included general physiological indexes,renal function parameters,histopathological changes and fibrosis-related factors.In terms of general physiological indexes,the body weight,body temperature,food intake and activity of the model group decreased,while the water and urine volume increased.In terms of renal function indexes,the contents of serum creatinine,urea nitrogen and uric acid increased,urine creatinine and urine protein/creatinine ratio increased,and osmotic pressure decreased in the model group.In terms of histopathology,pathological changes such as renal tubule dilation and extensive mild fibrosis were observed in the model group,and the MASSON-positive area ratio increased.In terms of fibrosis-related factors,the expression of transforming growth factor-β1(TGF-β1) in the kidney of mice in the model group increased,and the content of hydrogen sulfide decreased.The mouse model of chronic interstitial kidney injury established in this study is consistent with the disease characteristics of chronic interstitial kidney injury,and is suitable for the study of pathological mechanism and therapeutic drug evaluation of chronic interstitial kidney injury.