In order to better exploit and utilize oligosaccharides from Morinda officinalis How.,oligosaccharides from M. officinalis were isolated and purified from the herb powder of M. officinalis.The herb powder of  M. officinalis was first extracted by water bath reflux extraction to obtain the water extract part,and then separated by activated carbon column chromatography to obtain crude extract of oligosaccharide.Finally,eight oligosaccharides including kestose,kestostetraose,kestopentaose,kestohexaos,inulotriose,inulotetraose,inulopentaose and inulohexaose were obtained by twice preparation by gel purification system and once preparation by preparative liquid chromatography.The purity of the eight oligosaccharide monomers was tested by liquid chromatography,and their structures were identified by high resolution mass spectrometry,infrared spectroscopy and nuclear magnetic resonance spectroscopy.