In this study,UPLC-MS technology was used to detect the serum migration components of Stellera chamaejasme in rats with rheumatoid arthritis,and preliminarily evaluate the material basis,so as to laid a foundation for the further development and utilization of S. chamaejasme.After the chemical components of blank samples,medicated serum samples and in vitro samples (S. chamaejasme) were detected by UPLC-MS technology,the endogenous components in serum were removed from the medicated serum compounds,and then compared with the compounds obtained from in vitro samples.It is speculated that 19 serum migration components were detected under the positive ion mode in the drug-containing serum after oral administration of S. chamaejasme in rats.Among them,six components including bufalin,gelsemine,harmine,5-fluorouracil,malonic acid,and crocetin were related to the treatment of rheumatoid arthritis.Therefore,serum migration components of S. chamaejasme,including bufalin,gelsemine,harmine,5-fluorouracil,malonic acid,and crocetin may play a role in treating rheumatoid arthritis.