Abstract: Peroxisome Proliferator-Activated Receptor (PPAR) γ is one of the subtypes of PPAR,which are closely related to human chronic diseases,such as diabetes mellitus and the other metabolic diseases.In recent years,PPARγ has been as hot target for new drug development in treatment of diabetes area. Therefore,one type of high-throughput screening model for PPARγ was constructed at HeLa cell in present study,and the 58 different polar extracts from 12 kinds natural medicines were screened using this model.The results showed thatEtOAc extract (EB1),n-hexane extract (EA) and n-butyl alcohol extract from Edgeworthia gardneri (Thymelaeaceae) flower ,EtOAc extract (EB1) from Coptis Chinensis Franch of Sichuan, n-hexane extract (EA),supernatant of H2O extract (EC) and the remains of EtOH extract (EB3) from Pterocephalus hookeri (C.B.Clarke) Hoeck,and EtOAc extract (EB1) from Fructus Gleditsiae Abnormalis displayed the most remarkable activity for PPARγ,the maximum fold activation of 8 different polarity fractions compared to the positive control (0.5 μg/mL rosiglitazone),were 253%,199%,121%,127%,121%,109%,107% and 105%,respectively.This study provided the theories and experimental foundation for exploiting the novel,safe and high-efficiency natural medicines for the treatment of diabetes mellitus and its complication.

Key words: natural medicines;diabetes mellitus;Peroxisome Proliferator-Activated Receptor γ;PPAR&gamma, agonist