Abstract: One endophytic strain,coded SPS-02,was isolated from healthy stem of Artemisia annua L.On the basis of morphological character,culturing property and phylogenetic tree,strain SPS-02 was classified as Aspergillus fumigatus.The antimicrobial evaluation indicated that strain SPS-02 had strong inhibitory effect on phytopathogenic fungi,especially Alternaria alternate. Four secondary metabolites were isolated and respectively identified as bisdethiobis(methylthio)gliotoxin (1),N-(2,4-dihydroxybenzoyl)-L-serine (2),2,4-dihydroxybenzoic acid (3),2H-chromene-2,5(6H)-dione (4) using spectroscopic methods.Among these compounds,Compounds 1,3 and 4 were shown to inhibit Canidia albicans with MIC values of 0.90,2.08,1.98 μM respectively.By comparison with other secondary metabolites,the production of 1 was rich,the yield of which was as much as 15 mg/L,and had potential application in future.

Key words: Artemisia annua L., Aspergillus fumigatus;endophytic fungus;secondary metabolites;gliotoxin