Abstract: The aim of this study was to establish an HPLC method for simultaneous determination of salidroside,verbascoside,iso-verbascoside,leucoseptoside A,jionoside D and martynoside in Pedicularis rex, and to evaluate the anti-fatigue and anoxia endurance activities of P. rex extract.The chromatographic separation was carried out on a ZORBX C₁₈ (4.6 mm×150 mm,5 μm) column,with mobile phases:H₂O(A)-MeOH(B)(0~10 min,63% A;10~10.5 min,63%→58% A;10.5~40 min,58% A),at the flow rate of 1.0 mL/min and detection wavelength was set at 275 nm(0~4 min) and 330 nm(4~40 min).The column temperature was maintained at 35 ℃.The injection volume was 10 μL.The effective fractions of phenylpropanoids were prepared by macroporous adsorption resin.The contents of salidroside,verbascoside,iso-verbascoside,leucoseptoside A,jionoside D and martynoside had good linear relationship with in the ranges of 2.10~8.40,13.60~54.40,0.93~3.72,0.53~2.12,1.50~6.00,0.37~1.28,respectively,and the average recoveries of the 6 phenylpropanoids were all in the range of 98%~102%.The extraction yield of effective fractions was 0.62% with total contents of phenylpropanoids higher than 65%.The exhaustive swimming time and anoxia endurance time of three dosages groups were higher than those of the vehicle(P<0.01).The HPLC method was reliable,simple and accurate.It can be used for the quality control of P.rex. This experiment suggested that P.rex had the effect of promoting recovery,eliminating fatigue and improving exercise capacity.

Key words: Pedicularis rex, phenylpropanoids, HPLC, effective fractions, anti-fatigue, anoxia endurance