Abstract: To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of chlorogenic acid,neochlorogenic acid,nprotocatechualdehyde,nprotocatechuicnacid,cryptochlorogenic acid,caffeic acid,1,3-dicaffeoylquinic acid,ferulic acid,3,4-dicaffeoylquinic acid,3,5dicaffeoylquinic acid,4,5dicaffeoylquinic acid,aloe emodin,emodin and chrysophanol in Xanthii Herba and Xanthii Fructus.The assay was conducted on an Agilent ZORBAX SB-C₁₈ column (250×4.6 mm,5 μm) with methanol-0.1% formic acid in water as mobile phases.The flow rate was 1.0 mL/min,the detection wavelength was 254 nm and the column oven temperature was 35 ℃.The injection volume was 10 μL.The calibration curves of the 14 constituents showed good linearity (r>0.9931) within the ranges of the tested concentrations.The average recoveries (n=6) of 14 constituents were 97.18%-101.09%.The results showed that the contents of these 14 constituents varied in Xanthii Herba and Xanthii Fructus.The contents of chlorogenic acid and protocatechuic acid were the highest,while the contents of aloe emodin,emodin and chrysophanol were lowest.The developed method was simple,accurate and reproducible.It can be used for the quality evaluation and control of Xanthii Herba and Xanthii Fructus.

Key words: HPLC, Xanthii Herba, Xanthii Fructus, phenolic acids, anthraquinones, determination