Abstract: The α-glycosidase was extracted from the upper duodenum of Kunming mice.4-Nitrophenyl-α-D-glucopyranoside was used as substrate to establish a high throughput drug screening model of α-glycosidase inhibitor.A total of 2224 microbial fermentation extracts,including 1276 strains of actinomycetes and 132 strains of bacteria,were screened.23 positive samples were obtained,with a 1.03% positive rate at the first screening.The 23 strains were fermented and extracted for a second time,17 positive samples were obtained,with 3 bacterium and 13 actinomycetes.The positive rate was 0.74%.3 Positive samples were verified by α-glycosidase to have inhibitory effects higher than 75%.In conclusion,the developed α-glycosidase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs.

Key words: Alpha-glycosidase inhibitor, high-throughput screening model, microbial fermentation extract, Diabetes Mellitus