Abstract: To determine the contents of different chemical constituents in Polygoni Multiflori Radix(PMR)and Polygoni Multifori Caulis(PMC)from the same origin.The stilbenes,anthraquinones,flavonoids and phenolic acids in PMR and PMC were determined by UPLC-TQ/MS.the nucleosides were determined by QTRAP UPLC-MS/MS and the mineral elements were determined by ICP-MS.The results showed that the content of chemical constituents had large difference between PMR and PMC.In PMR and PMC,the stilbenes accounted for 26.135,15.307 mg/g,anthraquinones with 15.608,10.034 mg/g,flavonoids with 0.864,17.075 mg/g and phenolic acids with 0.529,0.720 mg/g.Furthermore,10 nucleosides accounting for 140.517,197.534 μg/g in both PMR and PMC.Among those nucleosides,guanosine made up for the highest percent with 55.688,90.273 μg/g.In addition,K was the most abundant mineral element in PMR and PMC with 12.538,16.747 mg/g,followed by Ca with 3.283,37.999 mg/g,Mg with 1.362,2.105 mg/g and P with 1.218,1.021 mg/g.This study provided a scientific basis for the comprehensive development of Polygoni Multiflori Radix and Polygoni Multifori Caulis.

Key words: Polygoni Multiflori Radix, Polygoni Multifori Caulis, stilbenes, anthraquinones, flavonoids, phenolic acids, nucleosides and nucleobases, mineral elements