Abstract: In this study, Larix gmelini sawdust was used as raw material for the purification of taxfolin with column process as the basic unit.With separation and purification as the purposes of the two units arranged in present technology,respectively,the column packed with AB-8 macroporous adsorption resins(MARs)and the preparative high performance liquid chromatography(prep-HPLC)were combined together.In the dynamic experiments carried out on AB-8 MARs column,the concentration of taxifolin contained in the sample solution was 0.5 mg/mL;the flow rate was 4 BV/h;the volume of the sample solutions was 169 mL,and the ratio of the diameter of the bed to the length of the bed was 1/10.In the eluting process,the flow rate was set at 3 BV/h.Five BV of deionized water was used for washing the adsorption column firstly.Then,8 BV of 20%(volume concentration)aqueous ethanol was used for eluting the separated products containing taxifolin.Through separation,the purity of taxifolin products was improved from 6.0% to 56.78%,with recovery of 88.71%.After being dried by the rotary evaporator,the separating products,in which the amount of taxifolin was 40 mg,were re-dissolved into 10 mL of 35%(volume concentration)aqeous ethanol to prepare the solutions for prep-HPLC unit.In this step,the eluting rate was 8 mL/min.The purity of taxifolin collected in this step was 95.02%.Setting the collection time range from 33.00 min to 40.50 min in the above method,the purity of taxifolin was further improved to 98.02%.

Key words: column process, Larix gmelini sawdust;separation;purification;taxifolin