NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2017, Vol. 29 ›› Issue (1): 1-6.doi: 10.16333/j.1001-6880.2017.1.001

Special Issue: No.3

• Article •     Next Articles

PTP1B Inhibitory Effect and Kinetics of Eremurus altaicus (Pall.) Stev.and Eremurus anisopterus (Kar.et Kir.) Regel Extract

XIE Lian-zhen1,2,3, XIAO Jia-qi1,2, LI Xun1,2, XIN Xue-lei1,2*, AISA Hajiakber1,2*   

  1. 1 Xinjiang Technical Institute of Physics & Chemistry,Chinese Academy of Sciences; 2 Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization Urumqi 830011,China; 3 University of Chinese Academy of Sciences,Beijing 100049,China
  • Online:2017-01-31 Published:2017-02-17

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Abstract: PTP1B plays an important role in the negative regulation of insulin signaling pathway.Overexpression of it leads to dephosphorylate the tyrosine residues of insulin receptor proteins,which is primarily responsible for insulin resistance in type 2 diabetes.Therefore,PTP1B are potential target for screening active ingredients of diabetes treatment.Inhibitory of PTP1B oriented,two specific Uygur herbs Eremurus altaicus and Eremurus anisopterus showed good inhibitory effect on PTP1B with IC50 of 17.85 and 33.76 μg/mL.The compound isolated from E.altaicus showed good inhibitory effect with IC50 of 26.15± 0.5 μM; The enzyme kinetic studies revealed mixed-type inhibition type with the inhibitory constants (Ki) of 39.12±8.56 μM.The study provided active guidance and theoretical basis for further isolation and purification of E.anisopterus.

Key words: T2DM, protein tyrosine phosphatase 1B, Uygur herbs, enzyme kinetic

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