Abstract: Online HPLCDPPH was used for determination of antioxidant active site from M.angusticeps.It mainly investigates the antioxidant activity of the aqueous extract、50% ethyl alcohol extract、95% ethyl alcohol extract was extracted by SPE,eluted with methanol，and chloroform extract.Based on this method,17 antioxidants have been found in M.angusticeps,among them,11 antioxidants have been found in aqueous extract、2 antioxidants have been found in 50% ethyl alcohol extract,and both of them showed high antioxidant activity.However,the results showed a minor constituent and not significant antioxidant activity in 95% ethyl alcohol extract and chloroform extract.M.angusticeps showed high antioxidant activity,the main components are polarity small molecular compounds,and are rich in aqueous extract.The antioxidants in M.angusticeps is well worth researching into.

Key words: HPLCDPPH, online detection, Morchella angusticeps, antioxidant activity