NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (Suppl1): 93-96. doi: 10.16333/j.10016880.2016.S.023

• Article • Previous Articles     Next Articles

Absorption Mechanism of Ziyuglycoside I in Human Caco2 Cell Monolayer Model

XIONG Yongai 1,DAI Liangmin1,YANG Guiyan1,ZOU Junbo 1,YANG Ming1,2*   

  1. 1 Pharmaceutical College,Chengdu University of Traditional Chinese Medicine,Chengdu 611173,China;2 Jiangxi University of Traditional Chinese Medicine,Key Lab of Modern Preparation of  Traditional Chinese Medicine,Ministry of Education,Nanchang 330004,China
  • Online:2016-06-30 Published:2017-03-02

Abstract: To study the absorption of Ziyuglycoside I in human Caco2 cell monolayer model.Caco2 cellmonolayer model was applied to investigating the bidirectional transport of Ziyuglycoside I.The effects of time,drug concentration,temperature,and inhibitor on the absorption of Ziyuglycoside I were observed.Drug concentration was measured by HPLC and the apparent permeability coefficients (Papp) were calculated.Time and concentration saturation were observed for the absorptive transport of Ziyuglycoside I across Caco2 monolayers.The transport of Ziyuglycoside I was influenced by the change of temperature and the presence of iodoacetamide,but not Verapamil or MK571.The absorption and transport mechanismof Ziyuglycoside I in Caco2 cell monolayers are active transportation mediated by transporter.

Key words: Ziyuglycoside I, Caco2 cell monolayer model, active transport, HPLC, mediated

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