Abstract: NDK105 is antibody of programmed death 1 receptor that has great potential in antitumor,antiinfection and autoimmune disease.We studied the effects of concentrate fedbatch culturing on rCHO growth,glucose consumption,as well as lactate and ammonia production.It is found that the maximum viable cell density in concentrate fedbatch culture reaches 3.1×106Cells/mL，which is 1.6fold in batch culture.At the same time,the culture time of rCHO were extended,and also,compared with the batch process,the newly developed concentrate fedbatch process reduced lactate production and improved NDK105 production.This concentrate fedbatch process would therefore facilitate the productions of therapeutic antibody by rCHO cells.

Key words: CHO cells, NDK105, lactate