Abstract: In this study,the microwaveassisted extraction (MAE) of Gynostemma pentaphyllum seeds oil was optimized.Based on single factor experiments,with yield of seeds oil as index,the extraction parameters (solid/liquid ratio,microwave temperature,microwave time and microwave power) were optimized by response surface methodology (RSM).The results showed that the optimal conditions to extract oil from G.pentaphyllum seeds were as follows:solid/liquid ratio of 1:15 g/mL,microwave temperature of 59 ℃,microwave time of 14 min,microwave power of 600 W.Under these conditions,the yield of G.pentaphyllum seeds oil (GPSO) reached (42.0085±0.02)%,which was close to the predicted value (41.2459%).The result showed that the developed method was feasible and reliable.

Key words: Gynostemma pentaphyllum, seeds oil, microwaveassisted extraction, response surface methodology