NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (增刊2): 234-238.doi: 10.16333/j.10016880.2016.S.010

• Article • Previous Articles     Next Articles

Optimization of Micro Screening Model for Acetyl Cholinesterase Inhibitors

SU Yongchang1*,LIU Shuji1,LIU Zhiyu1,YE Min2   

  1. 1Fisheries Reasearch Instiute of Fujian,National Research and Development Center for Marine Fish Processing (Xiamen),Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province;Fujian  Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361013,China; 2Minnan Normal University,Zhangzhou,363000,China
  • Online:2016-12-31 Published:2017-03-03

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Abstract: To optimize the micro screening assay for acetylcholinesterase inhibitors(AChEIs),wavelength,incubation time,substrate concentration,enzyme concentration and the solvent used in the screening were evaluated.Physostigmine and huperzine A were tested as positive drugs to verify the reliability of the model.Results showed that the micro screening model for AChEIs was optimized with wavelength 410 nm,incubation time 20 min,substrate concentration 4 mmol/L,AChE concentration 0.5U/mL.In addition,50% aqueous methanol was selected as an unified solvent to dissolve the samples in the screening.Physostigmine and huperzine A test indicated that the optimized micro screening system is stable,reliable and sensitive that can be successfully used to largescale screening of AChEIs.

Key words: acetylcholinesterase, inhibitor, microscreening model

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