Abstract: In this study,the effect of huperzine A on the expression of amyloid -β protein (Aβ) in the brain of transgenic mice was investigated.The levels of Aβ in wild type,APP/PS1 transgenic mice group and the treatment mice group were tested immunofluorescence.The expression of Bace1 and PS1 which are essential for β/γ-secretase activity was detected by Western blot and Real-Time PCR assays.It was found that the activity and content of Aβ in the huperzine A administration mice brain were decreased compared to that of the AD model mice,and the mRNA and protein expression levels of Bace1 and PS1were suppressed in the brain of huperzine A treatment mice group.These results prompted that huperzine A can decrease Aβ in AD mice brain through suppressing the activity of β/γ-secretase in APP/PS1 transgenic mice brain.These results suggested that huperzine A had important theoretical significance in the clinical treatment of AD.

Key words: huperzine A, APP/PS1 transgenic mice, A&beta, Bace1, PS1