Abstract: In mice MIR model,the myocardial infarct size,leaves of caspase3 mRNA and miR-140 were measured after scutellarin treatment.In cardiomyocytes MIR model,cell viability were assayed by MTT,the effects of scutellarin on caspase3 mRNA and protein expression of TLR4 and NF-κB were observed when miR-140 was inhibited or overexpression.The results showed that scutellarin can decrease the area of myocardial infarction,decrease the expression of caspase3 mRNA and increase the expression of miR-140 in vivo. In vitro, inhibition of miR-140 expression can counteract the anti-apoptotic effect of scutellarin,and increase the expressions of caspase3 mRNA,TLR4 and NF-κB.Overexpression of miR-140 reduced the expressions of caspase3 mRNA and TLR4 and NF-κB,which had a similar role to scutellarin.In conclusion,scutellarincan inhibit the apoptosis of myocardial cells induced by ischemia-reperfusion injury by upregulating miR-140 expression.

Key words: scutellarin, myocardial ischemia-reperfusion injury, caspase3, miR-140