Abstract: In this study,a comprehensive method was established to analyze and characterize the chemical ingredients in a new variety of Lonicera japonica by SPMEGC/MS coupled with HPLC-DAD.The volatile oils of the investigated sample were determined by GC/MS.Other organic acids,flavonoids and iridoids were analyzed by HSDE-HPLC-DAD.As a result,54 volatile oils were detected.In addition,the contents of four organic acids (chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A and isochlorogenic acid C),two flavonoids (rutin and galuteolin) and two iridoids (loganic acid and chiratin) were determined.This study proposed a method for the comprehensively and systematically evaluation of germplasm by determining the 4 types of main ingredients in L.japonica. It will provide scientific basis for the popularization and further utility of L.japonica.

Key words: GC/MS, HPLC-DAD, Lonicera japonica, volatile oil, organic acid, flavonoid, iridoid