Abstract: A HPLC method was established for the determination of 6 nucleotides and 3 amino acids in bamboo shoots.The detection condition was as follows:the column was YMC-Pack ODS-AQ C₁₈ (250×4.6 mm,5 μm),the mobile phase was acetonitrile (A) and 0.5% acetic acid in water (B),the flow rate was 1.0 mL/min, column temperature was 30 ℃ and the detected wavelength was 254 nm.Good linear relationship was achieved for the 9 targeted compounds within the concentration of 10-1000 μg/mL,the correlation coefficients all exceeded 0.999;the average recovery rate were between 86.74%-100.17% and the relative standard deviations were between 0.04%-2.90%.Method validation results showed that the developed HPLC method had good accuracy and precision.It can be used for testing the 9 targeted compounds in the bamboo shoots.

Key words: bamboo shoots, amino acids, nucleotides, quantitative analysis