Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity

doi:10.16333/j.1001-6880.2016.9.023

Abstract

Abstract: To investigate the preparation method on karenia polysaccharides with alkali protease enzymolysis from Karenia mikimotoi and its anti-angiogenic activity.The extraction of karenia polysaccharides was obtained by flash extraction combined with enzymolysis method,and the optimal process conditions were determined as enzymolysis temperature of 40 ℃,pH 8.5,enzymolysis dosage of 2.5% and enzymolysis time of 2 h.The karenia polysaccharides were obtained by using trichloroacetic acid to get rid of protein and ethanol for precipitation.The karenia polysaccharides showed some inhibition on the viability and migration of induced human umbilical vein endothelial cells at certain concentration.

Key words: Karenia mikimotoi, polysaccharides, flash extraction enzymolysis, anti-angiogenic activity

CLC Number:

O629.1

CHEN Yu-ting1,MENG Fan-tong1,2,KONG Liang1,TIAN Xi-fan1,TAN Cheng-yu1*. Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(9): 1470-1474.

[1]	YANG Xing-mao, YANG Cai-xia, ZHOU Xiao-tong, LIAO Zhen-yue, LIU Si-jing, GUO Jin-lin. Structural characterization of Cordyceps polysaccharides and their effects and mechanisms in improving efferocytosis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(8): 1288-1297.
[2]	ZHANG Kai-yue, ZHOU Yong-shun, HAN Kai, WANG Lu, CHEN Fu-long, GAO Jian-feng. Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp. GTD4C-2 and their antioxidant activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(7): 1112-1120.
[3]	ZHANG Hong-qi1, 2, ZHU Lie-qin1, ZENG Ya-ni1, CUI Min1, ZHOU Ying-xia1, DU Juan3. Optimization of extraction process of Phellinus lonicerinus polysaccharide and analysis of its antioxidant activity in vitro [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(4): 581-588.
[4]	CHU Si-yuan, QIAN Li-sheng, CHEN Hai-min. Research progress on the regulatory mechanism of tea components on gut microbiota and its health benifits [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(2): 357-367.
[5]	CAO Lei, LIU Wei, WANG Jun-long, NASEN Bate, BIEKETUERXUN Bahaerguli, SONG Bu-er, FENG Ya-ping, NUERXIATI Rehebati. Preparation of low viscosity polysaccharide from Plantago ovata Forsk by sulfation method and its structural characterization and antioxidant activities [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 26-36.
[6]	PAN Bo-ya, MENG Xian-qun, KANG Ting-guo, YIN Hai-bo, XU Liang, WANG Dan. Optimization of extraction process of Arctii Fructus polysaccharide based on response surface method and its anti-inflammatory effect on cells [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(1): 102-108.
[7]	LIAO Cai-yu, WU Li-yan, WANG Lan-ying, MENG Fan-xin. Research progress on the modulating effect of edible mushroom polysaccharides on alcoholic liver disease [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(增刊1): 165-174.
[8]	XU Hui-ying, DING Yi-hui, DONG Hao, ZHAO Xue-cen, BAI Yang, CAI Qian. Effects of polysaccharides and n-butanol fractions of Atractylodis Rhizoma on mitophagy in rats with spleen deficiency [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(8): 1372-1379.
[9]	ZHANG Ruo-bing, YANG Yu-he, LI Chen-xue, LI Wen-lan. Research progress on pharmacological action and mechanism of Ganoderma lucidum polysaccharides [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(5): 879-887.
[10]	HE Bao-feng, MA Xin-huan, MA Zhong-xiang, WANG Bao-cai, XU Zhi-wei, MA Fang-xiong, YANG Tian-yan, LI Rong-kun. Optimization of alcohol precipitation process for Glycyrrhiza uralensis polysaccharides based on improved entropy weight method combined with TOPSIS model and BPNN model [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(2): 271-280.
[11]	LIU Wen-juan, MA Shan-bo, LU Wen-fei, CUI Dong-xiao, XU Ding-qiao, YUE Shi-jun, CAO Wei, TANG Yu-ping. Isolation and purification of Qi-Gui polysaccharides and their in vitro inhibitory activity on tumor cell proliferation [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(10): 1651-1658.
[12]	ZHONG Cai-rong, CHEN Qin-chang, XU Han-yue, ZENG Zi-hang, XU Shao-si, CHE Dan-dan, FANG Jing-ping, HE Yong-jin. Effect of different light qualities on the growth and bioactive compounds biosynthesis of Isochrysis sp.ISO-FJ [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2023, 35(10): 1775-1781.
[13]	MENG Jing, HU Na, WANG Hong-lun. Activation of RAW 264.7 macrophages by the polysaccharide from the fruits of Nitraria tangutorum Bobr.and its molecular mechanisms [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(增刊1): 26-32.
[14]	GUO Hong, ZHANG Jing-nan, JIANG Li-li, YUAN Hong, XUE Xin, MA Chun-li, XU Song-yao, DENG Feng, XUE Hui-ting, HU Rui-ping. Optimization of extraction technology of polysaccharides from Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao by response surface methodology [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(增刊1): 78-83.
[15]	ZHANG Meng-yuan, YANG Xiao-hua, DUAN Meng-yue, ZHANG Hua-feng. Structural characterization of novel fraction EP80 of Epimedium sagittatum polysaccharides and its effects on enzymes related to alcohol metabolism [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2022, 34(9): 1539-1547.

Enzymatic Preparation of Karenia Polysaccharides and Its Anti-angiogenic Activity

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments