Abstract: To investigate the differences in accumulation and biosynthesis of metabolites in Pseudostellariae Radix. ¹H NMR based metabolomic approach combined with multivariate statistical analysis was used to investigate the chemical compositions and find the different metabolites in Pseudostellariae Radix from traditional field and cultivated fields.35 Metabdites were identified in the ¹H NMR spectrum,and 12 of which showed remarkable differences.The multivariate statistical analysis showed that Pseudostellariae Radix from cultivated fields and from traditional field can be separated obviously.The levels of arginine,glutamate,acetoacetate and succinate in Pseudostellariae Radix from traditional field were higher,while the cultivated Pseudostellariae Radix contained more lysine,dimethylglycine,phenylalanine,sucrose,xylose,quercetin,fumarate and ferulic acid.This study will provide the basic information for exploring the quality formation mechanism and revealing the differences in accumulation and biosynthesis of metabolites in Pseudostellariae Radix.

Key words: Pseudostellariae Radix, traditional field and cultivated fields;chemical compositions;¹H NMR;multivariate statistical analysis