Abstract: It is widely recognized that estrogen can improve the ability of learning and memory function.Former study have shown that EtOAc extract of Cynomorium songaricum (ECS)can improve both E2 level and the ability of learning and memory in ovariectomized rat,protect skn-sh cells from Aβ- injury.To contribute a better understanding of the mechanism of ECS that improve ability of learning and memory,we investigated the expression of synaptophysin and p-CREB in Neuro-2a cells.Using cck8 to detect the influence of ECS on Neuro-2a cells.Divided the cells into Normal group,DMSO group,ECS group Tamoxifen group,ECS+Tamoxifen group;Normal group,ECS group,ECS+G15 group and Normal group,DMSO group,ECS group,PD98059 group,ECS+PD98059 group.The results showed that ECS can significantly improve the expression of synaptophysin and p-CREB on Neuro2A cells.Treatment of the cultures with ER antagonist Tamoxifen+ECS cannot significantly decreased the expression of synaptophysin,but with GPR30 antagonist G15+ECS can significantly decreased the expression of synaptophysin.The antagonist of MAPK/ERK pathway anyagonist PD98059+ECS also significantly decreased the expression of p-CREB.According to preliminary experimental results,we hypothesized that ECS can improve the ability of learning and memory through MAPK pathway by combing with GPR30.

Key words: Cynomorium songaricum, p-CREB, synaptophysin, GPR30